Zyagen Bovine Testis Total RNA, 0.1mg

Bovine tissue Total RNA is extracted from freshly harvested tissues of single healthy normal donor using classical guanidine isothiocyanate-phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase-1 to remove residual DNA, precisely quantified, and stored at -80oC.Quality Control: The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. Applications: RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Packing/shipping/Storage: Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice. For long term storage, keep sample at -70oC.