Pyrophosphatase, Inorganic from baker's yeast (S. cerevisiae) (Inorganic Pyrophosphatase, PPi, Pyrophosphate phosphohydrolase), 227374-1Ku

Inorganic pyrophosphatase from baker's yeast is a homodimer consisting of two equal subunits of molecular weight 32-35kD. Inorganic pyrophosphatase catalyzes the following reaction: Pyrophosphate + H2 O ? 2- Orthophosphate This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present. The following metals can act as activators: Mg2+ > Zn2+ > Co2+ > Mn2+ > Ca2+. Molecular Weight: 71kD (homodimer consisting of two equal subunits of molecular weight 32-35kD) Extinction Coefficient: E1% = 14.5 (280nm) pI: 4.75 Solubility: ddH2O (2mg/ml) Unit Definition: One unit will liberate 1.0umole of inorganic orthophosphate/min at pH 7.2 at 25 °C. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.