Pigeon Nicotinic Acid Adenine Dinucleotide Phosphate ELISA Kit (ELISA Kit)

*Storage: Store all reagents at 2-8 degree C; *Accession#: O22261.2; O22261; *NCBI Summary: Encodes a chloroplast-localized NAD+ transporter that transports NAD+ in a counter exchange mode with ADP and AMP in vitro.; *Uniprot Summary: Function: Mediates the NAD+ import into chloroplast. Favors the NAD+(in)/ADP or AMP(out) antiport exchange, but is also able to catalyze a low unidirectional transport (uniport) of NAD+. Transports NAD+, nicotinic acid adenine dinucleotide, nicotinamide mononucleotide, nicotinic acid mononucleotide, FAD, FMN, TTP, TDP, TMP, UTP, UDP, UMP, CTP, CDP, CMP, GTP, GDP, GMP, 3'-AMP, ATP, ADP, AND AMP, has low transport activity with cAMP, pyrophosphate, NADH and alpha-NAD+, and has no activity with NADP+, NADPH, nicotinamide, nicotinic acid, adenosine, thiamine mono- or diphosphate, inorganic phosphate, CoA, folate, NaCl, malate, malonate, citrate, fumarate, aspartate, glutamate, S-adenosylmethionine, lysine, arginine, and ornithine. Ref.1 Ref.2Enzyme regulation: Inhibited by pyridoxal 5'-phosphate, bathophenanthroline, tannic acid, mersalyl, mercuric chloride, p-hydroxymercuribenzoate, p-hydroxymercuribenzoate sulfonate, bromocresol purple and N-ethylmaleimide. Ref.2Subcellular location: Plastid › chloroplast membrane; Multi-pass membrane protein Potential Ref.2. Tissue specificity: Highly expressed in young leaf mesophyll cells, root tips and at the branches of adventitious roots. Low expression in all flower tissues and not detected in siliques and seeds. Ref.2Miscellaneous: Appears to be a chloroplast envelope-located membrane protein lacking an N-terminal-located transit peptide.Sequence similarities: Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]Contains 3 Solcar repeats.Biophysicochemical propertiesKinetic parameters:KM=0.24 mM for for the NAD+/NAD+ exchange Ref.2Vmax=1.41 mmol/min/g enzyme for the NAD+/NAD+ exchange