PKA Activity Assay Kit

The B-Bridge cAMP-Dependant Protein Kinase A (PKA) Activity kit is designed to quantitatively measure PKA activity in Cell Lysate, Tissue Extracts and Buffer Samples and is species independent. The kit utilizes an immobilized PKA substrate bound to a microtiter plate. Samples containing PKA will, in the presence of the supplied ATP, phosphorylate the immobilized PKA substrate. A rabbit antibody specific for the phospho-PKA substrate binds to the modified immobilized substrate. Antibody specific for rabbit IgG labeled with peroxidase is simultaneously added to the plate to bind to the rabbit anti-phospho-PKA substrate. After a short incubation and wash, substrate is added and the intensity of the color developed is directly proportional to the amount of PKA in the samples and standards.