PGE2 Enzyme Immunoassay High Sensitivity kit (One Plate)

The Prostaglandin E2 (PGE2) Immunoassay kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, saliva and tissue culture media samples. A PGE2 standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse IgG. A PGE2-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to PGE2 to each well. After an overnight incubation, the plate is washed and substrate is added. The substrate reacts with the bound PGE2-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the PGE2 in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers. Serum, EDTA and Heparin plasma, urine, saliva and tissue culture media samples.