Histamine Receptor 3, CT (HR3) (Antibody)

*AppNotes: Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1-10ug/ml using ECL. ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well.; *Specificity: Recognizes rat Histamine Receptor 3 (HR3). The immunogen shows no significant sequence homology with other histamine receptors. Species CrossreactivityImmunogen: An18aa peptide sequence within the cytoplasmic, C-terminus of rat HR3 (KLH).; *Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.; *Accession#: NP_001153638.1; NM_001160166.1; Q9H3N8; *Description: Histamine, one of the most important mediators of allergy and inflammation, is a chemical messenger and aminergic neurotransmitters. It plays an important role in a multitude of physiological processes in central and peripheral tissues. Histamine is synthesized in a restricted population of neurons located in the tuberomammillary nucleus of the posterior hypothalamus implicated in many brain functions (e.g. sleep/wakefulness, hormonal secretion, cardiovascular control, thermoregulation, food intake, and memory formation). In peripheral tissues histamine is stored in mast cells, basophils, enterochromaffin cells. Histamine release leads to various well-known symptoms of allergic conditions in the skin and the airway system. Histamine effects are mediated by four pharmacologically distinct receptors, the H1R, H2R, H3R and H4R receptors, which are all members of the G-protein coupled receptor (GPCR) family. Histamine receptors display 7 TM domains, an extracellular N-terminus, and a cytoplasmic C-terminus of variable length. Human Histamine 3 receptor, H3R (GPCR97), has recently been identified as a presynaptic autoreceptor on histamine neurons in the brain controlling the...