Ferritin ELISA Kit

The Calbiotech Ferritin kit is a solid phase sandwich assay method, based on a streptavidin-biotin principle. The standards, samples and the biotinylated Anti-Ferritin antibody reagent are added into designated wells, coated with Streptavidin. Endogenous Ferritin in the patient's serum binds to the antigenic site of the biotinylated Anti-Ferritin antibody. Simultaneously, the biotinylated antibody is immobilized onto the wells through the high affinity Streptavidin-Biotin interaction. Unbound protein and excess biotin conjugated antibody are washed off by wash buffer. Upon the addition of the Peroxidase (HRP) conjugated Anti-Ferritin antibody reagent, a sandwich complex is formed, the analyte of interest being in between the two highly specific antibodies, labeled with Biotin and HRP. Unbound protein excess enzyme conjugated antibody reagent is washed off by wash buffer. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of Ferritin in the samples. A standard curve is prepared relating color intensity to the concentration of the Ferritin.