FSH ELISA

In the FSH ELISA Assay Kit ,the essential reagents required for an immunoenzymatic assay include high affinity and specificity antibodies (enzyme-linked and immobilised) with different and distinct epitope recognition, in excess, and native antigen. In this procedure the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti FSH antibody. Upon mixing monoclonal biotinylated antibody, the enzyme labeled antibody and a serum containing the native antigen, reation results between the native antigen and the antibodies without competition or steric hindrance to form a soluble sandwich complex. The interaction is illustrated by the following equation: Ka EnzAb(p) + AgFSH + BtnAb(m) ? EnzAb(p)- AgFSH- BtnAb(m) K-a BtnAb(m) = biotinylated monoclonal antibody (Excess quantity) AgFSH = native FSH antigen (variable quantity) EnzAb(p) = enzyme labeled policlonal antibody (Excess quantity) EnzAb(p)- AgFSH- BtnAb(m) = antigen-antibodies sandwich complex Ka = rate constant of association K-a = rate constant of disassociation Simultaneously the complex is deposited to the well through the high affinity reaction of streptavidin and biotinylated antibody. This interaction is illustrated below: EnzAb(p)-AgFSH-BtnAb(m) + streptavidincw ? immobilized complex Streptavidincw = streptavidin immobilized on well Immobilized complex = antibodies-antigen sandwich bound. | Sample Volume: 50 µl | Sample Type: Serum, Plasma | Assay Time: 1.5 hours | Species: Human | Regulatory Status: RUO