FOXD3 (Forkhead Box D3, HFH2, HNF-3 Forkhead Transcription Factor Genesis), F9046-10F-100ug

FOXD3 is a 58-60kD member of the winged helix transcription factor gene family. It is expressed in the epiblast, migrating neural crest, and neural progenitors of the developing spinal cord. FOXD3 is believed to maintain an uncommitted state in multipotent neural crest stem cells, and to induce cell cycle arrest via p53 and p21cip1. Human FOXD3 is 478aa in length. It contains a forkhead DNA binding domain aa141-235 plus an aa262-473. Over aa1-140 and aa302-462, human FOXD3 shares 76% and 91% aa identity with mouse FoxD3. Applications: Suitable for use in Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.