Edvotek 910 Isolation of Plant Mitochondria and Chloroplasts

In this two-part experiment, your students will explore the techniques used to isolate plant organelles. Two cell organelles are isolated from pea seedlings by differential centrifugation. First, students identify mitochondria by the enzyme activity of cytochrome c oxidase. Then, chloroplasts are isolated and identified under the microscope. Kit includes: instructions, chloroplast isolation reagents, mitochondrial DNA isolation reagents, transfer pipets, pea seeds. You need: microscope and slides, spectrophotometer, blender, waterbath, microcentrifuge tubes, clinical or high speed centrifuge (10,000 x g), pipet pumps bulbs, cheesecloth, distilled water, ice and ice bucket, acetone, vermiculite, nursery fat, automatic micropipets tips, lab glassware, scissors or razor blades, pasteur pipets, funnels, isopropanol, ethanol.