Edvotek 337 Drosophila Genotyping Using PCR, For 10 Lab Groups

Students will learn about DNA polymorphisms by amplifying DNA regions that vary between wild and mutant Drosophila. Amplified DNA from wild-type and white-eyed flies are separated by agarose gel electrophoresis and analyzed. Kit includes: instructions, PCR beads, primers, DNA extraction buffer, 200 bp ladder, Proteinase K, Drosophila (wild type and white eye), agarose, electrophoresis buffer and FlashBlue stain. All you need: thermal cycler, electrophoresis apparatus and power supply, automatic micropipet with tips, microwave, waterbath, UV transilluminator and microcentrifuge.