Chemglass CLS-4208-01 Glass 4.5mL Complete Hungate Anaerobic Culture Tube, with Screw Thread 16mm Diameter x 125mm Height, (Pack of 100)
The Hungate culture tube was developed for the cultivation of anaerobic microorganisms. These tubes simplify the anaerobic methods in several areas of microbiology. The technique is quite simple: Approximate 4.5mL of media is prepared under non-sterile conditions and gassed with oxygen free gas, then added to the gassed tubes. The rubber stoppers are inserted as the gassing needles are withdrawn and then capped. Once the tubes are autoclaved, they are inoculated with a syringe and incubated in a water bath.